Proteus vulgaris من Asparaginaseتنقية وتوصيف انزيم
Keywords:
Asparaginase, Purification, characterization, ProductionAbstract
The enzyme was extracted from Proteus vulgaris and partially purified by many steps including precipitation with ammonium sulfate by using different saturation percentages; the optimum percent was 80% gave value of specific activity 0.86 U/mg protien. then ion exchange chromatography by DEAE-cellulose column, the specific activity 2.7 U/mg protien, and gel filtration using Sephadex G-200 column specific activity 6U/mg protein. Characterization showed that the optimum pH and temperature for enzyme activity 7.5,37Downloads
Published
2012-12-01
Issue
Section
Articles
How to Cite
[1]
“Proteus vulgaris من Asparaginaseتنقية وتوصيف انزيم”, ANJS, vol. 15, no. 4, pp. 43–52, Dec. 2012, Accessed: Apr. 29, 2024. [Online]. Available: https://www.anjs.edu.iq/index.php/anjs/article/view/743